The Clarets head into the match with a four point gap separating them from Everton in 17th place, but a win over the Saints could pile the pressure on Frank Lampard's side as the battle to avoid relegation continues to twist and turn.
Meanwhile, Burnley are still on the hunt for a new manager, after parting company with Sean Dyche last week.
Speaking ahead of the game against Southampton, Burnley defender James Tarkowski said: “We need the backing (from the supporters). I know people might not agree with it (Sean Dyche's sacking) but only time will tell if it was the right thing to do. We still need the backing because the season is far from over, there are still a lot of points to play for, so we need them there at Turf Moor.
"It starts with two big home games this week. Sometimes a change can bring a little bit more freedom so hopefully it does change us and we start to perform a bit better and we get those results.
"We need to win (against Southampton), it's as simple as that. Draws aren't enough. We need three points on the board and we probably need three or four more wins to make sure we're safe at the end of the season.
"It's not just about individuals now, it's about that team mentality and spirit, which we've always had here and we've still got it. It's the time to show that now in the last few weeks of the season and it's really important to perform and get the results."
Take a look at the latest news and transfer stories from the Premier League, as the build-up to tonight's action continues.
1. Toffees could move for Wolves winger
Everton have been tipped to pursue a summer move for Barcelona man Adama Traore, as they prepare to lose star man Richarlison. Traore joined the Catalan giants on loan last January, but the Toffees could swoop if Barca decide not to make the temporary deal permanent. (Football Insider)
2. Hammers linked with Monaco defender
West Ham United have been linked with a move for AC Monaco defender Benoit Badiashile. The 21-year-old centre-back, who is valued at around £25m, is also believed to be on Serie A giants Juventus’ transfer radar. (Sport Witness)
3. Gunners-linked starlet could leave on the cheap
Arsenal could land Koln starlet Jan Thielmann for a fee of just £12.5m this summer. The Bundesliga youngster has shown glimpses of his talent in the German top tier this season, making eight starts and 18 appearances from the bench. However, the extent of the Gunners’ current interest is unclear. (Sport Witness)
4. Palace eye new midfielder
Crystal Palace are said to be talks with Lens’ over a move for their midfielder Cheick Doucoure. The 22-year-old Mali international is likely to cost around £16m, and his teammate Seko Fofana has described him as “incredibly complete” (HITC)