Burnley Ladies’ undefeated start to the league season ended in a 3-2 defeat at Chester City.
With several players unavailable, full backs Emma Culshaw and Saffron Hall made their debuts.
Chester dominated the early stages, but as the half progressed, Kelly Dean emerged as a creative force, crafting chances for Jo Rohman and Becca Sidley.
Burnley almost gained the lead on 20 minutes when a deft flick from Dean set Sidley free down the right, and her low cross picked out Charlotte Hartley, but her goal-bound shot was deflected.
The lively left winger Jane Sexton dispossessed the Chester full back in her own area, but her mis-hit shot faded wide of the post.
Against the run of play, Chester gained the lead on the stroke of half-time when their midfielder dribbled through to score a fine individual goal.
And shortly after the break when a defensive mix up allowed Chester to double their advantage.
But the lady Clarets dug in, and within 10 minutes managed to claw themselves level.
Dean flicked on a long throw and Sexton was first to react, rifling a right-foot shot past the keeper.
Then Dean found Claire Collins on the left, who transferred the ball onto her right foot and curled an unstoppable shot into the far corner of the net.
Chester regained the lead 20 minutes from the end with a fluent passing move, and Sophie Valentine kept Burnley in it with a fine penalty save.
Burnley Ladies are in cup action on Sunday at Skelmersdale.